phospho irf3 ser396 antibody  (Proteintech)

Journal: Materials Today Bio

Article Title: Biomimetic nanovesicle with tri-pronged immune amplification for efficient photo-immunotherapy against triple-negative breast cancer

doi: 10.1016/j.mtbio.2025.102641

Figure Lengend Snippet: CDN@PM-ICG-mediated STING pathway activation and ICD. (A) CDN@PM-ICG-mediated phosphorylation of TBK1 and IRF3 in BMDCs. (B) Quantative analysis of IFNb1, CXCL9 and CXCL10 mRNA expression levels in BMDC after different treatment using qRT-PCR. (C) The expression levels of IFN-β, TNF-α and IL-6 in the supernatant of BMDCs. (D) Representative images of immunofluorescence staining of CRT and HMGB1 in 4T1 cells. (E) Extracellular ATP content after indicated treatments. (F) The representative flow cytometry plots of (G and H) mature BMDCs (CD11c + CD80 + CD86 + ) and (I and J) M1-like macrophages (F4/80 + CD86 + ). The symbol “+” indicates laser irradiation.

Article Snippet: Anti-Phospho-IRF3 antibody (Cat. 4947S), anti-Phospho-TBK1/NAK antibody (Cat. 5483S) were offered by Cell Signaling Technology (Massachusetts, USA). β-Actin (Cat. AF7018) antibody and PD-L1 antibody (Cat. DF6526) were supplied by Affinity.

Techniques: Activation Assay, Phospho-proteomics, Expressing, Quantitative RT-PCR, Immunofluorescence, Staining, Flow Cytometry, Irradiation

Journal: The Journal of Headache and Pain

Article Title: Spreading depolarization activates the cGAS–STING pathway and drives cranial nociception: therapeutic potential of STING modulation

doi: 10.1186/s10194-026-02267-5

Figure Lengend Snippet: Expression of cGAS-STING pathway proteins in the ipsilateral parietal cortex increases following SD. (A) Schematic representation of the experimental design. (B) Representative immunofluorescence images showing STING, p-IRF3, and IFN-β expression in the ipsilateral parietal cortex of mice which underwent sham surgery, a single or six SDs. Scale bars: 50 μm. (C) Representative lane view images of capillary-based nanoimmunoassay (WES) analysis of STING, p-STING, IRF3, p-IRF3, and IFN-β protein levels in the ipsilateral parietal cortex following SD ( n = 4/group). D-H. WES analysis of STING, p-STING, IRF3, p-IRF3, and IFN-β protein levels in the ipsilateral parietal cortex following SD ( n = 4/group). Protein levels measured by WES were normalized to vinculin, a commonly used housekeeping protein, and the data are presented as fold changes relative to the sham group. Values are mean ± SEM. Statistical comparisons were made using Student’s t test with Welch’s correction. Not significant ( ns): p > 0.05, *: p < 0.05, **: p < 0.01, ***: p < 0.001. Panel A was created using BioRender.com

Article Snippet: The sections were washed with TRIS buffered saline (TBS) and blocked with 10% normal goat serum (Jackson ImmunoResearch, 005-000-121) at room temperature for 1 h. Next, sections were incubated overnight at +4 °C with primary antibodies: rabbit anti-STING antibody (1:200, monoclonal D2P2F, 13647 S, Cell Signaling Technology), rabbit anti-cGAS antibody (1:200, polyclonal, MBS9143559, MyBioSource), rabbit anti-p-IRF3 antibody (1:300, monoclonal, 29047 S, Cell Signaling Technology), rabbit anti-IFN-β antibody (1:300, polyclonal, NBP1-77288, Novus), rabbit anti-NLRP3 antibody (1:300, polyclonal, PA5115660, Thermo Fisher Scientific), rabbit anti-p65 antibody (1:200, monoclonal, 8242 S, Cell Signaling Technology), mouse anti-NeuN antibody (1:300, monoclonal MAB377 clone A60, Merck Millipore), goat anti-Iba1 antibody (1:250, polyclonal, NB100-1028, Novus), mouse anti-S100β antibody (1:200, polyclonal, Atlas Antibodies), rabbit anti-golgi autoantigen, golgin subfamily a, 2 (GOLGA2) antibody (1:500, polyclonal, 11308-1-AP, Proteintech).

Techniques: Expressing, Immunofluorescence